Biological Limnology Laboratory
The lab houses two environmental chambers for algal cultures and controlled experiments, equipment for isolation, identification and culturing including an autoclave, transfer hood, dissecting and inverted microscope equipped with epifluorescence and image analysis. A FacsCalibur flow cytometer is used for quantification of picoplankton. Nutrient analyses for most species of nitrogen, phosphorus and silica can be analyzed spectrophotometrically either manually or using the LaChat autoanalyzer. A FluoroProbe spectral fluorometer can be used in bench top mode or profiling mode to measure total chlorophyll and pigment composition and Walz PhytoPAM and Water PAM are used to assess photosynthetic capacity of algal cultures and discrete water samples. A Chelsea Instruments Fast Tracka II Fast Repetition Rate Fluorometer (FRRF) can be set up in the lab to measure variable fluorescence and absorption cross section of algal cultures and discrete samples and can be used in profiling mode in the field as well. An SAIV CTD with temperature, pressure, conductivity and oxygen sensors and a Satlantic hyperspectral radiometer completes the profiling instruments in the Biological Limnology Laboratory. In addition, the lab has a Walz Diving PAM, a Turner 10 AU fluorometer and a range of biological limnology sampling gear.